Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Gal3 inhibition reduced lung tissues damage induced by lung I/R (A) The Gal3 protein expression in lung tissue was assessed by western blotting, and the density of Gal3 (relative to β-actin) on western blotting was quantified (n = 3). (B–D) The expression of Gal3 in lung tissue, BALF and serum was detected by ELISA (n = 6). (E) Paraffin-embedded lung tissue sections were stained with hematoxylin and eosin (n = 3) at magnifications of 200×, scale bar 200 μm and 400X, scale bar 100 μm. (F) The extent of lung injury was assessed by scoring the F images (n = 6 section per group). (G) The W/D ratio of lung tissue (n = 6). (H) Ultrastructural changes were evaluated through transmission electron microscopy. Arrows indicate lamellar body and triangles mitochondria (n = 3). Scale bar, 1 μm. The data were mean ± SEM. One-way analysis of variance was used for data comparison, ns, not significant, ∗p < 0.05, was statistically significant.

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Inhibition, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Staining, Transmission Assay, Electron Microscopy, Comparison

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Gal3 inhibition attenuated the inflammatory response induced by lung I/R (A–C) BALF of left lung after lung I/R was collected, and the supernatant was extracted to detect the contents of TNF-α, IL-1β, and IL-10 in BALF. (D–F) After LIRI, the heart blood was taken, and the supernatant was collected by centrifugation after standing at low temperature for 3 h. The levels of TNF-α, IL-1β, and IL-10 in the serum were detected. (G–I) The lung tissue was subjected to standard treatment to detect the contents of TNF-α, IL-1β, and IL-10 in lung tissue. Data shown are mean ± SEM from n = 6 per group, ns, no statistical significance, ∗p < 0.05, was statistically significant.

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Inhibition, Centrifugation

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Inhibition of Gal3 reduces the expression of M1-type macrophages induced by lung I/R (A) Western blot of IL-1β, IL-6, TNF-α, and CCL5 in lung tissues. (B–E) Density analysis of IL-1β, IL-6, TNF-α, and CCL5 relative to β-actin in part A. (n = 3, mean ± SEM, ns, no statistical significance, ∗p < 0.05). (F and G) Lung tissues were stained with macrophages marker (F4/80, green), anti-IL-1β (red), and anti-IL-6 antibodies (red). Nuclei were stained with DAPI (blue). Magnification, 400×, scale bar, 100 μm.

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Inhibition, Expressing, Western Blot, Staining, Marker

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Inhibition of Gal3 increases the expression of M2 type macrophages induced by lung I/R (A) Western blot of Arg1, IL-10, Retnla, and Chil3 in lung tissues. (B–E) Density analysis of Arg1, IL-10, Retnla, and Chil3 relative to β-actin in part A. (n = 3, mean ± SEM, ns, no statistical significance, ∗p < 0.05). (F and G) Lung tissues were stained with macrophages marker (F4/80, green), anti-Arg1 (red), and anti-IL-10 antibodies (red). Nuclei were stained with DAPI (blue). Magnification, 400×, scale bar, 100 μm.

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Inhibition, Expressing, Western Blot, Staining, Marker

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Gal3 inhibition reduces macrophage polarization toward M1-type in OGD/R vitro model (A) Western blot of Gal3 in lung tissues. (B) Density analysis of Gal3 relative to β-actin in part A. (C) Cell viability was measured by CCK-8 assay (n = 6). (D) Western blot of IL-1β, IL-6, TNF-α, and CCL5 in RAW264.7 cells. (E–H) Densitometry of western blots in part D. Levels were standardized uniformly with β-actin. (n = 3, mean ± SEM, ns, no statistical significance, ∗p < 0.05). (I and J) Immunofluorescence staining showed macrophages marker (F4/80, green), IL-1β (red), and IL-6 (red) in RAW264.7 cells. Nuclei were stained with DAPI (blue). Magnification, 200×, scale bar, 200 μm.

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Inhibition, Western Blot, CCK-8 Assay, Immunofluorescence, Staining, Marker

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Gal3 inhibition promotes the polarization of macrophages toward the M2-type in OGD/R vitro model (A) Western blot of Arg1, IL-10, Retnla, and Chil3 in RAW264.7 cells. (B–E) Densitometry of western blots in part A. Levels were standardized uniformly with β-actin. (n = 3, mean ± SEM, ns, no statistical significance, ∗p < 0.05). (F and G) Immunofluorescence staining showed macrophages marker (F4/80, green), Arg1 (red), and IL-10 (red) in RAW264.7 cells. Nuclei were stained with DAPI (blue). Magnification, 200×, scale bar, 200 μm.

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Inhibition, Western Blot, Immunofluorescence, Staining, Marker

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet: Gal3 and TREM2 colocalized in macrophages and Gal3 inhibited the expression of TREM2 in LIRI (A) Macrophage markers (F4/80, green), anti-TREM2 antibody (red), and anti-Gal3 antibody (pink) were used for immunofluorescence co-localization staining of lung tissue. Magnification, 400×, scale bar, 200 μm. (B) Enlarge the dotted box in part A. F4/80 (green), TREM2 (red), and Gal3 (pink) co-localization qualitative analysis. White dots to white triangles area (white dotted line) were analyzed with line intensity scans at higher magnification. Measurement of the intensity values demonstrated co-localization in those regions. Magnification, 2000×, scale bar, 50 μm. (C) Western blotting and (E) RT-qPCR expression of TREM2 in lung tissue. (D) Western blotting and (F) RT-qPCR expression of TREM2 in RAW264.7 cells. (n = 3, mean ± SEM, ns, no statistical significance, ∗p < 0.05).

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Quantitative RT-PCR

Journal: iScience

Article Title: Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization

doi: 10.1016/j.isci.2023.107496

Figure Lengend Snippet:

Article Snippet: Gal3 ELISA kit , CUSABIO , Cat #CSB-E14296m.

Techniques: Recombinant, Modification, Saline, Protease Inhibitor, Enzyme-linked Immunosorbent Assay